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PGL3 Digestion

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#1 kabdelaz



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Posted 20 April 2010 - 06:11 AM

Iam just wondering if any one could help me, i have a problem with the digestion of PGL3 control vector, when i digested it using HindIII or BAMH i got 50% of it and also when i did the second digestion by using BgLII also i got 50% of the previous amount, and i didn't configure the problem with that. the second question is that how much i should use of PGL3 for TRansfection.
Thank you

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