Designing Primers for multiple Isoforms
Posted 20 April 2010 - 03:15 AM
To do this i have to use real-time PCR.
However, while i have the protocol for getting from cells to real time pcr, i have to design the primers.
And i have no clue how to approach this.
I would use something like pubmed to check the primer design, however the protein im characterising has 3 or 4 different isoforms, and i dont know how to design primers specific to a certain isoform.
Could anyone give me advice on how to do this?
Posted 20 April 2010 - 06:11 AM
Another option is to use Universal Probe Library Assay Design Center (it's primary for designing a UPL probe assay, but usualy you can use the primers for SYBR green without a probe) and let the aplication design a differentiation assay for selected isoforms.
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