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Finding fusion points in the genome by PCR


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#1 k8simmo

k8simmo

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Posted 20 April 2010 - 01:48 AM

Hi

Part of my research is looking at the fusion of short telomeres to other telomeres and other regions in the genome. I am trying to find a way to detect the fusion points in the genome which are unknown. Basically this would involve finding and sequencing a part of unknown sequence that is fused to known telomeric sequence. I have heard a bit about genome walking techniques such as inverse PCR and vectorette, but I am unsure of how they work and which would be best (if at all) for my project?

Any ideas/info would be greatly appreciated

Thanks!




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