Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to Improve EC50? EC50= Half maximal effective concentration


  • Please log in to reply
1 reply to this topic

#1 jamescorden

jamescorden

    member

  • Active Members
  • Pip
  • 14 posts
1
Neutral

Posted 18 April 2010 - 03:55 AM

We did an experiment for the MEASUREMENT OF AGONIST POTENCY which investigated the responses of a section of guinea-pig ileum (mounted in an organ bath) to acetylcholine and histamine.

How can we improve/obtain more reliable values of our EC50 value, given that we had time?? How


Any clues would be great. Thanks.

#2 Inmost sun

Inmost sun

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 303 posts
60
Excellent

Posted 20 April 2010 - 07:25 AM

We did an experiment for the MEASUREMENT OF AGONIST POTENCY which investigated the responses of a section of guinea-pig ileum (mounted in an organ bath) to acetylcholine and histamine.

How can we improve/obtain more reliable values of our EC50 value, given that we had time?? How


Any clues would be great. Thanks.



you have to re-done the experiment for several times, at least n = 3-times independently form each other which means using fresh preparations form other guinea pigs;

which effect of receptor stimulation are you measuring?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.