purified dna using dh5alpha and qiaprep....plasmid not in genome
Posted 16 April 2010 - 12:33 PM
Posted 16 April 2010 - 01:25 PM
Posted 18 April 2010 - 02:55 PM
Posted 18 April 2010 - 03:27 PM
When you search your sequencing result for your target sequence, depending on the sequencing primer's position and direction, you might get reads on only partial sequence or the complementary sequence. Make sure that you don't miss it because of those. Use blast2n tool to compare your sequencing result to your target sequence.