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more than one band


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#1 ARR

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Posted 16 April 2010 - 11:28 AM

hellow every one,

i had a problem in my results, when i do the RT-PCR for Norovirus the electrical cable of the thermal cycler was not fixed, so the reaction was stopped twice before i fix the cable tightly.

this is the picture for agarose gel electrophoresis, can any one suggest why i have more than one band?

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#2 chrisbelle

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Posted 22 April 2010 - 02:52 AM

hellow every one,

i had a problem in my results, when i do the RT-PCR for Norovirus the electrical cable of the thermal cycler was not fixed, so the reaction was stopped twice before i fix the cable tightly.

this is the picture for agarose gel electrophoresis, can any one suggest why i have more than one band?


the other samples seem fine, maybe it's contamination for that one sample? I assume you mean the first lane on the left after ladder...
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