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Mol bio Equipments for lab


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#1 smilealie

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Posted 16 April 2010 - 05:08 AM

Hello every one,

We have to set up a new molecular biology lab and we are looking for the all type of equipment for the lab.those how have worked in this field can help me out i think.Kindly provide the instrument name so that it will be help full for the new lab setup.Dnt think of budget it may be toooooo costly or it may be cheap.Kindly tell me the instruments which are used.kindly suggest.

thanks in advance,
alie.

#2 bob1

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Posted 19 April 2010 - 04:49 PM

At a minimum you will need a microcentrifuge (refrigerated), water baths, a MilliQ water supply, fridges and freezers (-20 and -70, NOT frost free), micropipettes (e.g. eppendorf, gilson, biohit), measuring cylinders, volumetric glassware, bottles, beakers, flasks, a fume hood, a pH meter, stirrer plates, and balances for weighing things.

Other equipment will depend on what you are wanting to do... PCR machine, agarose gel apparatus, acrylamide gel apparatus, power supplies for running gels, a gel documentation system, film cassettes, microscopes, cell culture facilities (bacteria and/or eukaryote), incubators (shaking and normal, CO2 injected etc...), autoclave, film processor, vacuum evaporator, a spectrophotometer (conventional or nanodrop style), microplate reader, Real time PCR machine...

Really the list is huge, you should find an already set up lab that is doing techniques that you want to do and go around the lab writing a list of what they have (and what they would like to have as well), then work out how much each is going to cost, and how that will fit with your budget.

A site like Biocompare.com will be pretty useful for you. However, if you go to manufacturers and are buying as lot of things from them, you can often get a discount and free shipping.

Also don't forget small items like scissors, personal protective equipment, lab coats, tape dispensers, folders for protocols, lab books, spatulas, stirrer magnets, etc...

#3 smilealie

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Posted 19 April 2010 - 05:50 PM

hi bob,

thanks for your list.i need to know particularly molecular cell biology equipments.i have written all small things.but i need to know something as you said like real time pcr ,flow cytometry like this some good useful higher end instrument.if you have any idea kindly let me know.

thanks,
alie.



At a minimum you will need a microcentrifuge (refrigerated), water baths, a MilliQ water supply, fridges and freezers (-20 and -70, NOT frost free), micropipettes (e.g. eppendorf, gilson, biohit), measuring cylinders, volumetric glassware, bottles, beakers, flasks, a fume hood, a pH meter, stirrer plates, and balances for weighing things.

Other equipment will depend on what you are wanting to do... PCR machine, agarose gel apparatus, acrylamide gel apparatus, power supplies for running gels, a gel documentation system, film cassettes, microscopes, cell culture facilities (bacteria and/or eukaryote), incubators (shaking and normal, CO2 injected etc...), autoclave, film processor, vacuum evaporator, a spectrophotometer (conventional or nanodrop style), microplate reader, Real time PCR machine...

Really the list is huge, you should find an already set up lab that is doing techniques that you want to do and go around the lab writing a list of what they have (and what they would like to have as well), then work out how much each is going to cost, and how that will fit with your budget.

A site like Biocompare.com will be pretty useful for you. However, if you go to manufacturers and are buying as lot of things from them, you can often get a discount and free shipping.

Also don't forget small items like scissors, personal protective equipment, lab coats, tape dispensers, folders for protocols, lab books, spatulas, stirrer magnets, etc...



#4 bob1

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Posted 21 April 2010 - 05:02 PM

For real time I only really know of ABI (Invitrogen just bought the company) and Roche light cyclers, out of which I would go for the Roche instruments - expensive but work really well.

For FACS, I guess Beckmann Coulter are the people there.

Microplate readers - We have a Fluostar from BMG labtech, which is pretty good, but there are lots of different companies that do different types.

#5 mdfenko

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Posted 23 April 2010 - 10:34 AM

for real time there is also biorad.
talent does what it can
genius does what it must
i do what i get paid to do

#6 smilealie

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Posted 27 April 2010 - 06:05 PM

Thanks for every one for suggesting the equipments.




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