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Copy nr calculation


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#1 Jka83

Jka83

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Posted 16 April 2010 - 03:37 AM

This text is from a article (methods part), Wang et al. Jour of Clin Microb Sept 2000

"Since
2 ml out of 50 ml of RNA eluates, which were derived from 140 ml of culture
supernatant or plasma, was used in each reaction, the number of dengue virus
RNA copies per reaction was divided by 5.6 ml (140 ml 3 2 ml/50 ml) and
multiplied by 1,000 to obtain the number of RNA copies per milliliter of supernatant
or plasma."

I may be stupid but I just donīt understand the reasoning here? Could someone clear this a bit for me?

Cheers,

"Stupid lab technician"




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