The following formula is from vectorlab DNA, RNA 5 end labeling kit a and b are clear but C wasn't sure about it?
If I have for example an RNA of 700 kb how calculate it?
use the following formula to calculate
nmols of 5' ends in the nucleic acid to
A = the concentration of nucleic acid (μg/μl)
B = average molecular weight of nucleotide
(333 μg/μmol for DNA; 317 μg/μmol for RNA)
C = total number of bases (for single-stranded
DNA or RNA) or the number of base pairs (for
cAN ANYBODY HELP TO SOLVE THIS PROBLEM
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