Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

cAN ANYBODY HELP TO SOLVE THIS PROBLEM


  • Please log in to reply
No replies to this topic

#1 Meral

Meral

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 15 April 2010 - 11:07 PM

Dear all,

The following formula is from vectorlab DNA, RNA 5 end labeling kit a and b are clear but C wasn't sure about it?
If I have for example an RNA of 700 kb how calculate it?

use the following formula to calculate
nmols of 5' ends in the nucleic acid to
be labeled:
A
BxC
where:
A = the concentration of nucleic acid (μg/μl)
B = average molecular weight of nucleotide
(333 μg/μmol for DNA; 317 μg/μmol for RNA)
C = total number of bases (for single-stranded
DNA or RNA) or the number of base pairs (for
double-stranded DNA).





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.