Solublising Casein for use as blocking agent
Posted 03 July 2002 - 04:04 AM
Posted 05 July 2002 - 08:44 AM
I use alkalisoluble casein (Merck) for blocking agent in western blot ( QIAGEN protocol).
casein preparation :
- make TBS + 1% casein pH 7,5 as :
1) tris 10mM + Na Cl 150mM + casein without adjust pH (pH 10) with stirring.
2) Adjust pH with HCl 1N at pH 7,5
3) stir at least 30mn and adjust the final volume
Posted 12 July 2002 - 03:53 AM