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microglia staining


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#1 Velella

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Posted 15 April 2010 - 07:48 AM

Hello,

I am looking for a protocol to stain microglia on paraffin embedded brain and spinal cord sections.

I would prefer a fluorescent method because I need to do a double staining and also take some photos with confocal microscopy.

Thank you in advance, any help will be greatly appreciated.

Valeria

#2 fablastar

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Posted 01 June 2010 - 11:16 AM

I used ED1 from abdserotec.
up to 1/500 but works in fluo best at 1/200 on rat brain tissue.
classic protocol




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