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mycoplasma detection- soft agar assay


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#1 labrat612

labrat612

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Posted 15 April 2010 - 05:44 AM

Hi guys!

As some of you may know, the FDA regulations stipulate doing mycoplasma detection using Soft Agar Assays and Hoescht Staining.

I'm trying to implement these protocols in the lab that I am in. Does anyone have a good protocol for these assays? We have both suspension-adapted cells as well as adherent cells.


Thanks.
Maya




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