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functionality of yeast promoter in E. coli


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#1 kajmak

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Posted 15 April 2010 - 12:58 AM

Is it possible that yeast ADH1 promoter is working in E. coli?
thank you

#2 phage434

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Posted 15 April 2010 - 05:53 AM

Not in the same way. There may be promoter activity of a DNA region which is a yeast promoter, but it will not be because the E. coli recognizes the same signals as yeast does. There could be accidental promoter activity, since bacterial promoters are quite degenerate.




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