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[SF_HK]

Hi,

I need to collect protein lysate to detect bax protein levels by western following 48 hour transient transfection of my gene of interest. I have a question regarding transent transfection.

24 hours following transfection, do I change medium in the 6 well plates to complete medium or medium containing g418?

[tea-test]

for transient transfection you do not select resistant cells by exposure to an antibiotic like G418. that's why it is only transient.