Problems with small peptide electrophoresis
Posted 14 April 2010 - 09:14 AM
I've tried running it in a 6%, 8%, 12%, 17% native gel. I've tried using 1xTBE running buffer at both pH 8.5 and pH 10, in case the calculated PI (7.3) was wrong. I've tried visualizing with both Coomassie and silver staining after fixing the gel with glutaldehyde in case it was a problem with the visualizing methods. I've also tried running it on a SDS gel. Still no protein. I've done LC-MS to ensure that the peptide isn't degraded. I've done BCA to make sure I had peptide in my eppendorfs. I'm at my wit's end. Does anyone have any other ideas I should try?
Posted 14 April 2010 - 10:49 AM
Posted 16 April 2010 - 08:36 AM
what is the range of your standards? are they native or sds standards?
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