Affinity purifed Polyclonal, better than monoclonal for IP?
Posted 14 April 2010 - 03:09 AM
I am using the Direct IP kit from Pierce for immunoprecipitation, with 2ug of affinity purified polyclonal antibody coupled to the amino resin.
This antibody is very expensive and we were wondering if it should be replaced by a cheaper monoclonal antibody.
The kit protocol simply explains that the antibody must be affinity-purified and be free of serum proteins.
It is equivalent a monoclonal antibody to a highly purified polyclonal?
Posted 15 April 2010 - 12:22 PM
genius does what it must
i do what i get paid to do
Posted 15 April 2010 - 08:05 PM