I need to do a growth curve on some Cal27 and our lab is having a debate on the best way to do it. I was planning on using a 96 well plate and adding the same amount of cells to each well and then trypsinizng and counting the cells with a hemocytometer. The other way would be to use flasks but that would involve many flasks and more media. Any advice is appreciated on the best way to do this.
I come from a bacterial background and mammalian cells are new to me.
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bob1
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Posted 13 April 2010 - 04:20 PM
96 well plates won't have enough cells in each well for an accurate count of the cells by haemocytometer. I would suggest wells of a 24 or 6 well plate at a minimum if you are going to lift and count.
You could do something like an MTT assay that measures the number of viable cells based on absorbance of a dye compound. All you have to do is make sure that you set up appropriate time points for collection of the data.
You could do something like an MTT assay that measures the number of viable cells based on absorbance of a dye compound. All you have to do is make sure that you set up appropriate time points for collection of the data.
