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Stimulating hepatocytes with IL-1beta


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#1 kellyly

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Posted 13 April 2010 - 09:45 AM

Hi,

I'm wondering if anyone knows of a generic concentration of IL-1beta that could be used in a stimulation experiment on hepatocytes? I am using Hep89 cells, and I want to stimulate in 6-well plates with estradiol-only and estradiol with IL-1beta. I wouldn't be sure on a concentration, no time to do a conc curve and papers only give values at 100U/ml which is no good to me as the protein I have is at 25ug/ml.
Any suggestions?

Thanks
L

#2 tea-test

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Posted 14 April 2010 - 12:53 AM

hi,

I don't know if you have seen this publication, maybe its helpful:

Le Vee M, Gripon P, Stieger B, Fardel O. Down-regulation of organic anion
transporter expression in human hepatocytes exposed to the proinflammatory
cytokine interleukin 1beta. Drug Metab Dispos. 2008 Feb;36(2):217-22. Epub 2007
Nov 8. PubMed PMID: 17991769


it seems that they have used 1ng/ml IL-1beta.
tea-test: The artist formerly known as Ned Land




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