Posted 13 April 2010 - 09:23 AM
Briefly my protocol is:
- 2ug of DNA in 32.5ul of water + 1.1ul of 10N NaOH (freshly prepared) - incubate @ 55C for 15 mins
- Add 1ul of glycogen; 1.5ul of hydroquinone + 200ul of bisulphite (freshly prepared; ph is 5)
- Incubate at 50C for 16hrs or 55 for 4hrs
- Clean up using qiagen kit
Any suggestions would be welcome!
Posted 14 April 2010 - 04:21 AM
how are you assessing conversion has worked there?