Has anyone had success using additives in sanger sequencing reactions to remove sequence fragment migration anomalies/artefacts
0
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 2,240 posts
an elder
77
Excellent
Excellent
Posted 15 April 2010 - 12:03 PM
what kind of artifacts?
what are you seeing?
on what are you visualizing the reaction (machine, gel,...)?
some standard pcr additives (eg- betaine, ethylene glycol, propylene glycol, dmso, etc) work with sequencing difficult templates.
what are you seeing?
on what are you visualizing the reaction (machine, gel,...)?
some standard pcr additives (eg- betaine, ethylene glycol, propylene glycol, dmso, etc) work with sequencing difficult templates.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
