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Removing sequencing migration artefacts


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#1 DavidS

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Posted 13 April 2010 - 06:39 AM

Has anyone had success using additives in sanger sequencing reactions to remove sequence fragment migration anomalies/artefacts

#2 mdfenko

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Posted 15 April 2010 - 12:03 PM

what kind of artifacts?

what are you seeing?

on what are you visualizing the reaction (machine, gel,...)?

some standard pcr additives (eg- betaine, ethylene glycol, propylene glycol, dmso, etc) work with sequencing difficult templates.
talent does what it can
genius does what it must
i do what i get paid to do




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