Edited by bochum, 13 April 2010 - 06:08 AM.
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What is the minimum DNA concentration requried for double digestion?
1 reply to this topic
Posted 13 April 2010 - 09:43 AM
You need about 10 ng to see a band, so you need about 20x that much (8000/400) or 200 ng of DNA in your digestion, if you run the entire digestion. A standard digestion of 1 ug in 50 ul would be a good start, running 20 ul on a gel.