Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Preparing competent cells under laminar flow?


  • Please log in to reply
5 replies to this topic

#1 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,112 posts
59
Excellent

Posted 13 April 2010 - 03:36 AM

Hi all,

I've always made my chemically competent cells myself and I always prepare them on my bench. until the last part of addition of glycerin.

I prepare TOP10 and on the invitrogen protocol there is no sign of preparation under laminar flow. It's common sense to prepare under laminar flow to have the least contamination, but I've never had any problem so far.

my friends say my TOP10 might get contaminated with other bacteria and not work properly after a while.

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,410 posts
234
Excellent

Posted 13 April 2010 - 09:56 AM

I think there are very very few things you need a laminar flow hood for in working with bacteria, other than to protect yourself from pathogens. Your bench should be just fine.

#3 HomeBrew

HomeBrew

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 930 posts
15
Good

Posted 13 April 2010 - 06:12 PM

Over the last twenty years, I've made countless batches of chemically competent E. coli. Never once did I use (or, to be honest, even think about using) a hood. You'll be fine...

#4 virusfan

virusfan

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 32 posts
0
Neutral

Posted 14 April 2010 - 08:38 AM

I also never prepare mine in the hood, even the last step. Everything is done on bench. :(

#5 Inmost sun

Inmost sun

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 303 posts
60
Excellent

Posted 16 April 2010 - 03:19 AM

Hi all,

I've always made my chemically competent cells myself and I always prepare them on my bench. until the last part of addition of glycerin.

I prepare TOP10 and on the invitrogen protocol there is no sign of preparation under laminar flow. It's common sense to prepare under laminar flow to have the least contamination, but I've never had any problem so far.

my friends say my TOP10 might get contaminated with other bacteria and not work properly after a while.


There is no need to use a flow; the risk to contaminate the flow with bacteria is higher than to spoil your preparation;

(in some labs it is done when they have more laminar flow capacity than normal benches)

#6 tea-test

tea-test

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 169 posts
18
Good

Posted 16 April 2010 - 07:04 AM

if someone is preparing bacteria in my laminar flow hood i will kick him out of the window :D
tea-test: The artist formerly known as Ned Land




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.