Posted 12 April 2010 - 04:52 PM
Can some one please clarify this for me since I;m getting confused. I have a gene which is 2.7kb in length and it is the open reading frame of the gene that I;m interested in studying. This is in a plasmid that has an ars (autonomous replication sequence), but Iím really not sure about the location of the promoter. I think it is under the own promoter of the gene, but based on the DNA sequence of the gene can I find where the promoter is?
I;m really confused.
Posted 14 April 2010 - 07:59 AM
Or the plasmid can have own (usualy viral) promoter upstream of the ligation site. That should be denoted in the plasmid map.
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