I need some help I did a singleplex and a multiplex for CTX-M-1 and 9 and I got different lengths when compared to a 1kn ladder (promega). I am not reall sure what length they are supposed to be now.
If anyone could let me know that be great.
My gel results with 1kb ladder:
M9= 900 bp
Multiplex (CTX-M-1,2,9 were used in the reaction)
M1= 450bp region
M9= 200bp region
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CTX-M-1 and CTX-M-9 Bp
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