Sample Grinding For DNA extraction
Posted 09 April 2010 - 08:12 AM
I am looking into a cryomill by Retsch or a Spex 6870 Freezer/Mill. The container that hold the sample are generally Stainless Steel and after grinding could potentially give off trace elements such as Fe, Cr, Ni, Mn, S, Si (<0.02% for Si).
With the exception of Mn affecting chelator concentrations, can anyone comment on the possible downstream effects on PCR or QPCR reactions.
I currently grind in N2 with a mortar and Pestle.
Posted 11 April 2010 - 04:08 PM
Posted 12 April 2010 - 10:17 AM
Posted 12 April 2010 - 07:13 PM