I started to do some work with Gel shifts and am getting results where the free probe runs at a certain point on the gel and when i add whole cell extract the band move higher in the gel and higher still as the conc. of WCE added increases. I don't see a shift between a lower free probe form and a higher bound probe form that I would expect just this slower migration as [WCE] increases. The control which is WCE of a culture not expressing my protein of interest does not shift or move the band when the [WCE] increases.
Has anyone seen this before and does anyone know what i shappening to the probe?
Edited by rsphorler, 09 April 2010 - 07:48 AM.