What is the minimum amount (ng)o f protein that can be detected by SDS and WB?
basic question in SDS and western blot
1 reply to this topic
Posted 09 April 2010 - 04:46 PM
I would imagine as little as 1-5ng can be detected but this must be a function of your antibody too. Better monoclonal antibodies, especially for epitope tags like HA and FLAG detect much better than polyclonal protein specific antibodies. Hope this helps.