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ubiquitin isopeptidases


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#1 botanicus

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Posted 09 April 2010 - 12:21 AM

Hi! Do you know if there are any comercially available enzymes which could cleave ubiquitin from monoubiquitilated protein.
I've read a loy of articles but couldn't found something reasonable :blink:

Maybe somebody knows how could I detect ubiquitination besides Western and MS?

#2 sumogirlie

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Posted 09 April 2010 - 04:39 PM

I really think you need to Western to confirm Ub. You can try a proteasome inhibotor like MG-132 to see if your protein accumulates, instead of a direct Ub ab.

MS will only map your Ub sites.

Biomol/Enzo Life Sciences makes a bunch of SENP and Ulp type enzymes.

#3 botanicus

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Posted 12 April 2010 - 05:50 AM

I really think you need to Western to confirm Ub. You can try a proteasome inhibotor like MG-132 to see if your protein accumulates, instead of a direct Ub ab.

MS will only map your Ub sites.

Biomol/Enzo Life Sciences makes a bunch of SENP and Ulp type enzymes.



;) thanks a lot




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