2 replies to this topic

[Sapphire.Waters]

I coated my glass coverslips with PDL and gelatin, dried them, stored them in a sterile culture dish in the dark at room temperature. Now I'm worried they may be contaminated and would like to sterilize them.
Is it possible to autoclave already-coated glass coverslips?

[rhombus]

Sapphire.Waters, on Apr 8 2010, 10:23 AM, said:

I coated my glass coverslips with PDL and gelatin, dried them, stored them in a sterile culture dish in the dark at room temperature. Now I'm worried they may be contaminated and would like to sterilize them.
Is it possible to autoclave already-coated glass coverslips?

The answer really is NO....

The best way to sterilise them would be by Gamma Irradiation....or to just re-coat some fresh coverslips

Hope this is useful

Kindest regards

Rhombus

[Sapphire.Waters]

Thanks, that's what I figured. tow that I think about it, it's possible that PDL-gelatin lattices probably would've locked in the contamination I would've wanted to remove. I'm best off just making fresh coverslips.
Thanks anyway, figured it was worth a shot.