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Autoclaving coated glass coverslips?


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#1 Sapphire.Waters

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Posted 08 April 2010 - 01:23 AM

I coated my glass coverslips with PDL and gelatin, dried them, stored them in a sterile culture dish in the dark at room temperature. Now I'm worried they may be contaminated and would like to sterilize them.
Is it possible to autoclave already-coated glass coverslips?

#2 rhombus

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Posted 08 April 2010 - 03:41 AM

I coated my glass coverslips with PDL and gelatin, dried them, stored them in a sterile culture dish in the dark at room temperature. Now I'm worried they may be contaminated and would like to sterilize them.
Is it possible to autoclave already-coated glass coverslips?



The answer really is NO....

The best way to sterilise them would be by Gamma Irradiation....or to just re-coat some fresh coverslips

Hope this is useful

Kindest regards

Rhombus

#3 Sapphire.Waters

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Posted 08 April 2010 - 11:39 PM

Thanks, that's what I figured. tow that I think about it, it's possible that PDL-gelatin lattices probably would've locked in the contamination I would've wanted to remove. I'm best off just making fresh coverslips.
Thanks anyway, figured it was worth a shot.




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