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PCR additives Formamide


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4 replies to this topic

#1 biomolpal1

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Posted 08 April 2010 - 01:07 AM

Hi,
I was using a 50% formamide from a Sigma Optimization kit II, and it really was beneficial to improve my multiplex PCR.
Running out of this reagents, we decided to use a in house formamide (Highly deionized usually used in sequencing gel) which did not work out...then we ordered 100ml Formamide from Sigma (after consulting the UK and Europe customer service)...and again it was more detrimental to my PCR than anything else.
The EUrope customer service told us that it was the only product available but that it was not the same as in the PCR OPT kit, because it was a deionized formamide!

anybody knows about formamide as an additive to PCR? product out there that are adapted to PCR optimization? where could I find it??

thanks a lot,
any comments, answer to help would be great!

Edited by biomolpal1, 08 April 2010 - 03:58 AM.


#2 Adrian K

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Posted 08 April 2010 - 08:49 AM

Try DMSO and BSA.... or combination of both.
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#3 tea-test

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Posted 08 April 2010 - 10:06 AM

hi i have found a supplier here, its formamide designated for PCR: http://www.qbiogene..../pcr/dmso.shtml

but i don't know if it works.
tea-test: The artist formerly known as Ned Land

#4 phage434

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Posted 08 April 2010 - 11:13 AM

Or try 3-6% of a 1M betaine solution (my favorite).

#5 biomolpal1

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Posted 12 April 2010 - 02:07 AM

Thanks a lot for that,
I think I migth try it!




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