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Sankella, on Jan 13 2010, 09:04 AM, said:

I am planning to do a dual luciferase assay using the firefly and renilla luciferase vectors.
Can someone suggest me a ratio for the co-reporter vectors to be added to the transfection mix.

Regards
Sankella

StevieRay, on Jan 13 2010, 09:13 AM, said:

Your best bet is to first test them at different ratios, say ranging from 1 luc: 1 ren to 1000 luc: 1 ren. The reason is that luciferase and renilla constructs can have different activity, depending on the cell line you transfect them into. Renilla will vary some (up to 10-50 fold depending on the cell line and the promoter it is driven by), while luc can vary considerably, depending on your promoters activity in the cell line you are testing.