Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

PCR Master mix


  • Please log in to reply
1 reply to this topic

#1 Bassaml7

Bassaml7

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 40 posts
0
Neutral

Posted 07 April 2010 - 03:40 AM

Hi,

I am working on population genotyping for a certain polymorphism. Since there are many samples, I am trying to make things as quick and easy as possible. So first I am preparing a master mix. I just wonder if it's ok to prepare a master mix that contains the primers as long as Taq is not included.

The master mix I am preparing will also be 1X meaning that I will just divide the prepared mastermix into 45 ul aliquots and just add the template DNA and Taq. So the master mix would contain the same concentration of dNTP ,PCR buffer and primers as the final PCR reaction.

The master mix in itself will be divided into few aliquots. These aliquots are expected to be used in less than 15 days. The aliquots will be stored at -20.

Also , my PCR includes 5 % DMSO , is it ok to be incorporated into the mastermix with the other ingredients ?

Thank you in advance.

#2 gebirgsziege

gebirgsziege

    I like fungi

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 392 posts
25
Excellent

Posted 07 April 2010 - 04:50 AM

I have repeadedly stored ready for use aliquots (19 L) of MM containg taq for up to a week at -20C and never had any problems.
A man cannot be too careful in the choice of his enemies. (Oscar Wilde)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.