3 replies to this topic

[PCR Novice]

Hi All,

There is a PhD student in our lab who is thinking about doing some PCR work with zebrafish embryo's (72hours post fertilization). I have one quick question, does anyone have any idea how many embryos she might need to get a good yield of RNA-oh the other problem is she will be using primers specific for expression in the liver.

Thanks  

[swanny]

PCR Novice, on Apr 7 2010, 07:32 PM, said:

Hi All,

There is a PhD student in our lab who is thinking about doing some PCR work with zebrafish embryo's (72hours post fertilization). I have one quick question, does anyone have any idea how many embryos she might need to get a good yield of RNA-oh the other problem is she will be using primers specific for expression in the liver.

Thanks  

40-50 should be plenty.

[Helios]

I get ~30ug RNA from 40-50 72hpf embryos.

[PCR Novice]

Thanks a million that will be a great help