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RNA from Zebrafish


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3 replies to this topic

#1 PCR Novice

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Posted 07 April 2010 - 01:32 AM

Hi All,

There is a PhD student in our lab who is thinking about doing some PCR work with zebrafish embryo's (72hours post fertilization). I have one quick question, does anyone have any idea how many embryos she might need to get a good yield of RNA-oh the other problem is she will be using primers specific for expression in the liver.

Thanks :lol:

#2 swanny

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Posted 07 April 2010 - 04:17 PM

Hi All,

There is a PhD student in our lab who is thinking about doing some PCR work with zebrafish embryo's (72hours post fertilization). I have one quick question, does anyone have any idea how many embryos she might need to get a good yield of RNA-oh the other problem is she will be using primers specific for expression in the liver.

Thanks :lol:

40-50 should be plenty.
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#3 Helios

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Posted 08 April 2010 - 03:25 AM

I get ~30ug RNA from 40-50 72hpf embryos.

#4 PCR Novice

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Posted 08 April 2010 - 06:43 AM

Thanks a million that will be a great help




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