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CMV-delta R8.2 and pCMV-delta R8.2


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#1 windson007

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Posted 07 April 2010 - 12:48 AM

Hi all,
Anyone can tell me whether the two plasmids, CMV-delta R8.2 and pCMV-delta R8.2, are the same? If not, which one is fit for producing lentivirus?

#2 Functional Screens

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Posted 07 April 2010 - 04:42 PM

They should be the same. They were derived from R9 by deleting Env and other HIV accessory genes. This is 2nd generation Gag-Pol maker and good for lentiviral vector with wild-type LTR.

#3 miBunny

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Posted 13 April 2010 - 05:01 PM

Second generation systems will also package third generation lentiviral vectors (in addition to second generation vectors).
This one should express TAT which is required for all second generation vectors and dispensible for third-gen

Edited by miBunny, 13 April 2010 - 05:02 PM.


#4 Functional Screens

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Posted 14 April 2010 - 02:12 PM

Second generation systems will also package third generation lentiviral vectors (in addition to second generation vectors).
This one should express TAT which is required for all second generation vectors and dispensible for third-gen

True, in my experiences using the 2nd generation packaging system seems not as good as using the 3rd generation system for 3rd generation lenti vector in virus production.




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