what is the measurement of Oil red O staining
Posted 06 April 2010 - 06:26 AM
I'm doing oil red O staining of macrophages,but what is the measurement of ORO staining?
The number of cells stained by ORO?
The fluorescense intensity of ORO?
Or comparing the red area in cells?
Posted 06 April 2010 - 04:23 PM
Posted 06 April 2010 - 04:45 PM
Posted 07 April 2010 - 01:58 AM
I am going to compare the degree of foam cell formation between 2 groups of mouse peritoneal macrophages.
As I know,there are 2 measurements for this:
1) ORO staining
2) Quantitation of intracellular contents of cholesterol
Are there any else ways?
For oil red O staining,is OD/mg protein suitable?
or fluorescence intensity?