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Stripping temperature


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#1 goldfinger

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Posted 04 April 2010 - 05:52 AM

Hi all,
Our lab does not have a working water bath now. And I wonder if I can use room temperature to strip my WB membrane? or 37C?

I use home made stripping buffer:

62.5mmol/l Tris PH6.7
100mmol/l beta-mercaptoethanol
2%SDS

Thanks!

#2 sumogirlie

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Posted 09 April 2010 - 04:58 PM

I'd try it at 37C if that's all you have. But if you have a floor shaker incubator that you can adjust the temp on somewhere in your building that would be good too.

If you're desperate try making your own water bath, but I don't know how long you strip for. I almost never strip, just run another gel!




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