Posted 04 April 2010 - 02:21 AM
does anyone have experience with double-immunofluorescent staining with primary antibodies raised IN THE SAME SPECIES!?
Would be very grateful for useful tips how to handle this and whether it is at all advisable.
Posted 06 April 2010 - 05:32 AM
Posted 06 April 2010 - 04:34 PM
Posted 09 April 2010 - 03:21 AM
We used the same protocol, worked well once we had it optomised but optomising did take a bit of time. It makes a difference which primary/secondary combo you use first so try both ways, and as Bob1 said, make sure the secondary antibody steps are saturating or you'll be in a world of frustration!
Edited by squallweathered, 09 April 2010 - 03:22 AM.