Constitutively Ubiquitination Mark?
Posted 03 April 2010 - 10:45 AM
Now, i need to know if an ubiquitin mimic exists. I am do not know of the DUB for the particular protein I'm studying.
Any suggestions? Thanks.
Posted 04 April 2010 - 04:43 PM
Posted 06 April 2010 - 01:09 PM
I thought it will only blot for any ubiquitin present on a protein. I am looking to see if there is a such a thing as a ubiquitin-mimic like a phosphomemitic. I know in some of my experiments i'll be using an acetylation mimic by substitutin lysine residues for glutamate residues to mimic acetylation.
Posted 06 April 2010 - 07:03 PM
To be honest, I don't know if there is (are) such mimicking replacement(s), as for phospho- and acetyl-mimetics. But I've read some articles in which they replaced Leu/Ile by Ala in order to inhibit the UIM. Besides, there is also one study that showed that Ser residues flanking ub-Lys enhaced its ubiquitination (http://www.pnas.org/...03/13/4958.full).
I'm not quite sure if it's exactly what you're looking for, but I hope your experiment works fine.
Btw, if you find out such a residue substitution that leads to increased Ub, please, let me know.
Good luck there!