Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

trouble sterile filtering fibrinogen solution

  • Please log in to reply
No replies to this topic

#1 erinrun3



  • Members
  • Pip
  • 1 posts

Posted 02 April 2010 - 01:14 PM

Hi all,

I'm having trouble sterile filtering a 200 mg/ml solution of bovine fibrinogen solution. It simply won't go through a .22 uM filter. I've tried to "step down" through several filters from larger to smaller filter sizes, without success.

Sigma says the fibrinogen solution needs to be dissolved in 0.85-.9% NaCl. I've used both DMEM (.64% NaCl) and F10 (.74% NaCl) to no avail. The fibrinogen solution takes about 4 hours at 37 degrees C to dissolve. The solution is clear after 4 hours, so it's clearly dissolving to some extent, just not enough.

Any suggestions? This problem is killing this project. Thanks.


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.