Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

conventional v real-time PCR applications


  • Please log in to reply
1 reply to this topic

#1 Ljw6

Ljw6

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 02 April 2010 - 10:15 AM

Couple of questions for those who know...

In general what type of testing (both research and clinical) is done using conventional qualitative PCR? real-time/qPCR?

Do people still use conventional PCR much? who and for what?

In clinical applications is infectious disease testing typically done using conventional or real-time?

Thanks for replies!!

#2 dreww

dreww

    RNA Follower

  • Active Members
  • PipPipPipPipPip
  • 36 posts
0
Neutral

Posted 05 April 2010 - 02:30 AM

Real time PCR seems to be the route to go nowadays due to it's ability to detect low copy #s. However, the cost of real time is a bit higher due to machine itself and reagents such as specific dyes and what not. conventional pcr is still used for simple detection methods or molecular techniques such as gel extractions. both are still used, however it depends on the applications and or downstream applications.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.