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Primer Design in 3´non translated version vs. coding region


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#1 bluedoozer

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Posted 01 April 2010 - 11:28 AM

Hello to all of you researcher out there,

I have a general question about Primer design. I´ve seen that many researcher design primers in the 3´untranslated region of a gene´s mRNA. Why????????
Why not using the CDS of the mRNA?????? with exon /intron boundaries !

Has anyone a good explanation?

Thanks in advance

bluedoozer

#2 pcrman

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Posted 01 April 2010 - 06:38 PM

I don't think that is a good idea because 3UTRs are more variable than coding region.

#3 bluedoozer

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Posted 01 April 2010 - 10:31 PM

I don't think that is a good idea because 3UTRs are more variable than coding region.


You are probably right, but many researchers are doing this. I check a lot of primers from publications and a lot of them were located in the 3´UTR regions. And so I´m wondering why




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