Primer Design in 3´non translated version vs. coding region
Posted 01 April 2010 - 11:28 AM
I have a general question about Primer design. I´ve seen that many researcher design primers in the 3´untranslated region of a gene´s mRNA. Why????????
Why not using the CDS of the mRNA?????? with exon /intron boundaries !
Has anyone a good explanation?
Thanks in advance
Posted 01 April 2010 - 06:38 PM
Posted 01 April 2010 - 10:31 PM
I don't think that is a good idea because 3UTRs are more variable than coding region.
You are probably right, but many researchers are doing this. I check a lot of primers from publications and a lot of them were located in the 3´UTR regions. And so I´m wondering why