PCR reagents, can I use reagents from different manufacturers?
Posted 31 March 2010 - 06:46 PM
I am mainly doing clinical screening using nested PCR in my lab.
And I am wondering whether would it be OK to use reagents from different manufacturers,
ie using Taq from Invitrogen and dNTP from Fermentas.
Would doing so have any effect on my results?
Any input is greatly appreciated!
Posted 31 March 2010 - 07:29 PM
Posted 01 April 2010 - 12:18 AM
as i dnt run clinical type of reaction bt once i face different band intensities from different sources of taq.
Posted 23 January 2015 - 04:14 AM
Posted 23 January 2015 - 03:41 PM
Reagents are well-characterized chemicals. As long as you make sure that you are substituting with the same chemical (complex ones in some cases), it should make no difference what kits you mix and match. Enzymes may have some different characteristics, so make sure you are using same name enzyme, but otherwise you can always make adjustments to your protocol and do some trial and error. Hey, you may end up making a better kit!
So. Now that you have your first ever question on bioforum answered (or not), mail yourself your username and password so you don't forget them, and then come back soon to update us on how it all worked out. That's how you build Karma in science.