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Antigen Binding B-cell

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#1 anonymous



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Posted 17 February 2001 - 10:00 PM

Does anyone have a protocol for fluorescent staining of immune mouse splenocytes with the antigen conjugated to FITC or some other fluorescent marker?I've done studies in the past looking at B-cell proliferation and T-cell populations in immunized mice. I haven't found any protocols using a conjugated antigen.Thanks.Nanci

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