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Can I redo a linearization

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#1 PCR Novice

PCR Novice


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Posted 31 March 2010 - 03:19 AM

Hi All,

Got a bit of a problem. I was linearising a plasmid, cutting with BamH1 in one tube and Xba1 in the second (making sense and antisense probes) but I made a silly mistake and mixed up the buffers. Can I clean up the mix and isolate the plasmid again and redo???

Any help would be brilliant

Cheers :rolleyes:

#2 phage434



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Posted 31 March 2010 - 05:46 AM

Yes, your DNA is still there. It might even be cut correctly. Most restriction enzymes will cut even if it is the wrong buffer.

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