1 reply to this topic

[PCR Novice]

Hi All,

Got a bit of a problem. I was linearising a plasmid, cutting with BamH1 in one tube and Xba1 in the second (making sense and antisense probes) but I made a silly mistake and mixed up the buffers. Can I clean up the mix and isolate the plasmid again and redo???

Any help would be brilliant

Cheers

[phage434]

Yes, your DNA is still there.  It might even be cut correctly.  Most restriction enzymes will cut even if it is the wrong buffer.