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Detection of phospho-proteins


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#1 Halfro22

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Posted 30 March 2010 - 08:29 PM

Hi everyone,

I am trying to detect a changes in a phospho-protein that is in small abundance in my sample. Normally I have been running 20 ug per lane but have recently read that when detecting for phospho-proteins it is best to run a small amount of sample in order to detect differences across groups. This leads me to think that I should run 5-10 ug protein per lane. Does anyone have any thoughts on this? BTW I read this in some other forum and can't remember where I found it!

#2 In_search_of_truth

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Posted 19 June 2010 - 02:41 PM

Hi everyone,

I am trying to detect a changes in a phospho-protein that is in small abundance in my sample. Normally I have been running 20 ug per lane but have recently read that when detecting for phospho-proteins it is best to run a small amount of sample in order to detect differences across groups. This leads me to think that I should run 5-10 ug protein per lane. Does anyone have any thoughts on this? BTW I read this in some other forum and can't remember where I found it!


How are you detecting your phospho-proteins? are you using a gel staining kit or are you using an antibody?




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