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Hybridization signal and detection p-value


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#1 Mr. Naive

Mr. Naive

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Posted 30 March 2010 - 09:33 AM

Hi folks,

Back again with some stupid question :( I am working with illumina microarray data using beadstudio software to study differential expression of various miRNAs in 4 groups of samples (total 12 samples).

My question is - to identify the "significant" over and under expression of miRNA in different groups what criteria should be used. Literature showed using >2 fold increase in signal as over expression and <0.5 fold as under expression when comparison is made between two groups. Also used along with that is significant p-value.

But is the criteria p-value, the detection p-value of each hybridization signal intensity? or can we get a different p-value for the comparison between 2 groups, which is not given in the final report of the array; if so how to get it?

Thanks in advance,
MN




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