I am using an anti-acetylated tubulin antibody for my western blots and I just wanted to know what the exact epitope it binds to. From what I've read previously, it may bind to the acetylated Lysine 40 residue, but if this is the case, wouldn't the antibody pick up acetylated lysine residues on other proteins besides the tubulin? My guess is that there is a specific epitope, in addition to the acetylated lysine group, that the anti-acetylated tubulin antibody binds to. Please let me know if you have any ideas.
Edited by Aravin, 30 March 2010 - 07:14 AM.