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membrane protein aggregation


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#1 handsome

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Posted 28 March 2010 - 05:12 PM

I expressed a membrane protein in insect cell . The expression level is reasonable. At low concentration, the protein is quite stable and the fplc profile is good. But once at high concentration, more than half of the protein went to aggregate.

Is there anybody could give me some suggestion how to improve it ?

Thanks!

#2 mdfenko

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Posted 29 March 2010 - 10:19 AM

add something that may prevent aggregation (eg ethylene glycol, peg-8000 1-5%) and limit concentration.
talent does what it can
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