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SDS-PAGE revelation problems


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#1 Granovski.V

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Posted 26 March 2010 - 11:31 PM

Hello all.

Im having a trouble whit SDS-PAGE revelation, my gels become yellow or some times dont reveal at all.
In my laboratory all of us are experiencing this problem, some times the revelation is good and white and some times is yellow or dont reveal. The revelation buffers protocol is the same for all. and the method also.
any ideas?

#2 mdfenko

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Posted 29 March 2010 - 09:55 AM

the protocol is the same (what is your protocol?) but are you using the same solutions as your labmates or making them as necessary?

are you loading enough sample?

can you post a picture of your gel?
talent does what it can
genius does what it must
i do what i get paid to do




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