What are the disadvantages of using Sodium borate buffer for DNA electrophoresis?
How high we can go with the voltage for mini gels?
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#2
pDNA
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Posted 25 March 2010 - 10:22 PM
you can run them at 200V, they should be done in 10-15 mins (use 10-35 volts/cm).
Regards,
p
Regards,
p
#3
hsb31
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Posted 30 March 2010 - 02:29 AM
pDNA, on Mar 26 2010, 11:52 AM, said:
you can run them at 200V, they should be done in 10-15 mins (use 10-35 volts/cm).
Regards,
p
Regards,
p
Thanks for the reply. I have read that if one use standard loading dye, resolution is not good at higher voltage (200-250V).
Is it fine to use this buffer, when doing gel elution also.
